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How to Run a 2-D Electrophoresis Gel from Start to Finish

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In this video, Steve Freeby of Bio-Rad Laboratories provides complete, step-by-step user instructions for two-dimensional (2-D) protein electrophoresis using IPG strips and polyacrylamide gels. Topics covered include how to apply samples and rehydrate the IPG strips, how to equilibrate the IPG strips prior to running on the second-dimension gel, and staining and visualizing proteins on the finished gel. Practical tips are given at each step to ensure high-quality results using Bio-Rad equipment and reagents.

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