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Creation of murine excisional wound splinting model and transplantation of stem cells

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All animal experiments were performed with the approval of the Animal Ethics Committee of Tsinghua University. Splints are prepared from a silicon sheet. After anesthesia, two equal sized full-thickness wounds are created on the depilated dorsal skin of a Balb/C mouse with a biopsy punch. Stem cells are injected into the dermis around the wound. Spread an instant-bonding adhesive on one side of a splint and carefully place the splint around the wound so that the wound is centered within the splint. Secure the splint to the skin with interrupted sutures. Apply stem cells in Matrigel onto the wound bed. Cover the wounds and splints with sterile transparent dressing Tegaderm. Dress the wounds with self-adhering elastic bandage. Full protocol described in:

Xusheng Wang et al. The mouse excisional wound splinting model, including applications for stem cell transplantation. Nature Protocols 8, 302--309 (2013) doi:10.1038/nprot.2013.002

http://dx.doi.org/10.1038/nprot.2013.002

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Fashion converse, inc.We wanted people to recognize us as http://description.bluebase-cms.com/content/does-go-michael-kors-hobo-bag-uk-down being in a band when we walked down the street, even if they didn't know which band we were.Chuck taylors signaled that. "

Our country apparently doesn't want low income americans to have free access to birth control, either by compelling all insurance plans to offer it or by adequately funding public reproductive health programs.In many schools predominantly located in low income, high teen pregnancy.

Our country apparently doesn't want low income americans to have free access to birth control, either by compelling all insurance plans to offer it or by adequately funding public reproductive health programs.In many schools predominantly located in low income, high teen pregnancy. (Catherine rampell)

If you want to project an image, you gotta have a style.For footwear, pumps say something.So do wingtips.And so do converse chuck taylor sneakers:Put on some chuck taylors and a pair of skinny jeans and you look like someone ready to strap on a guitar.

But what exactly is it about these shoes or any fashion item that allows them to express personality?That's the crux of an aggressive trademark infringement action brought by converse that also raises a broader question:Why do we choose to dress as we do?

If you aren't sure about chucks, picture the high top sneakers with the rubber toe cap worn by old time basketball players like wilt chamberlain, who scored 100 pointsInone http://www.snccf.org/and-michael-kors-online-outlet-1040s/ game while wearing them.In"West side story,"The sharks wore them.InRocky, Sylvester Stallone trainedInthem.

In the mid 1970s, chuck taylor sneakers started showing up on the feet of underground rock bands(Blondie, the dead boys, members of the ramones), giving cheap canvas shoes the street fashion cachet of an expensive leather motorcycle jacket.Athletes don't wear chucks anymore, but musicians still do, and so do kids who want to look cool, and certain misguided baby boomers who think it's still possible they can look cool.

Converse generates about $1.7 billion in sales, according to The New York Times, and wants to protect its brand.So the company, owned by nike, has lashed out at dozens of competitors, alleging they infringe on converse trademarks by selling knockoff versions of chuck taylors.

Some of the competitors' shoes look a lot like chucks.But winning these lawsuits won't be easy.Fashion is more than fickle, it's an elusive attribute, making it sometimes difficult to describe why some shirts are more attractive than others, or why exactly chuck taylors are unique.

Under trademark law, converse will have to meet a high standard http://www.monicacurtopelle.it/index.php?option=com_content&view=article&id=63 to protect its design because, really, sneakers are sneakers.The company will have to prove the chuck taylor look is specifically associated with the brand in the mind of the consumer. "It can't just be that consumers like your design,"University of pennsylvania law professor r.Polk wagner told the times. "It can't just be that your design is different or novel or interesting. "

This freedom given to manufacturers to riff on each other is great for shoppers.It's the reason that high fashion trends turn up so quickly on the racks of discount chains.But at some point borrowing can become stealing.Luxury designer christian louboutin, known for its red sole shoes, successfully sued yves saint laurent for copying that attribute.

Chuck taylors have a specific look, including the toe cap and bumper plus an upper stripe and a lower stripe.To anyone with a rock 'n' roll sensibility, they have a powerful allure.Kurt cobain wore them.So do members of green day.

"Chuck taylors had that retro trash americana thing, and punk rock style was about going back to the '50s and http://autned.ru/?p=458 pretending hippies didn't happen,"Arturo vega, the ramones' artistic director, told spin.

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