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Creation of murine excisional wound splinting model and transplantation of stem cells

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All animal experiments were performed with the approval of the Animal Ethics Committee of Tsinghua University. Splints are prepared from a silicon sheet. After anesthesia, two equal sized full-thickness wounds are created on the depilated dorsal skin of a Balb/C mouse with a biopsy punch. Stem cells are injected into the dermis around the wound. Spread an instant-bonding adhesive on one side of a splint and carefully place the splint around the wound so that the wound is centered within the splint. Secure the splint to the skin with interrupted sutures. Apply stem cells in Matrigel onto the wound bed. Cover the wounds and splints with sterile transparent dressing Tegaderm. Dress the wounds with self-adhering elastic bandage. Full protocol described in:

Xusheng Wang et al. The mouse excisional wound splinting model, including applications for stem cell transplantation. Nature Protocols 8, 302--309 (2013) doi:10.1038/nprot.2013.002