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How to punch lab mice ear

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The ear punch in this video:
The hole position map.

Different types of ear punches are available.

Ear punches should be sterile prior to initial use.

Extra ear punches should always be available, as they become dull with repeated use.

The punch should be placed approximately 3 mm from the edge of the ear pinna.

If the punch is placed too close to the edge of the pinna, it is likely to tear and become difficult to read.

You should also be careful not to place the punch too far towards the inside of the ear to avoid injuring the animal.

The tissue removed with the ear punch can be used for genotyping.

Sanitize the ear punch between each cage of animals with 70% ethanol.

Using a chlorinated compound will cause the punch to become corroded.

Re-sterilize the instrument after use.