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Freezing Cells: GIBCO® Cell Culture Basics

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An established cell line is a valuable resource and its replacement is expensive and time consuming. It is therefore vitally important that the cell line is frozen down and preserved for long-term storage. The best method for cryopreserving cultured cells is storing them in liquid nitrogen in complete medium in the presence of a cryoprotective agent such as dimethylsulfoxide (DMSO). Cryoprotective agents reduce the freezing point of the medium and also allow a slower cooling rate, greatly reducing the risk of ice crystal formation, which can damage cells and cause cell death. This video demonstrates the critical steps required to freeze cells while maintaining optimal cell health. We review the equipment required, how to prepare for freezing and each step performed in a careful way at the right pace to prevent damage to your cells.